Amplification methods

Details Amplification methods Amplification methods

2008-01-15

2008-01-15

Kim, Young J. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120

Reexamination Certificate

active

07319022

ABSTRACT:
Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.

REFERENCES:
“Designing TaqMan® MGB Probe and Primer Sets for Gene Expression Using Primer Express® Software Version 2.0”, pp. 1-15, [online] [retrieved on Aug. 17, 2007], retrieved from http://keck.med.yale.edu/affymetrix/rtpcr/quantitative/Probes%20and%20Primer%20Design%20using%20primer%20express.pdf.
Afonina et al. Minor Goove Binder-Conjugated DNA Probes for Quantitative DNA Detection by Hybridization-Triggered Fluorescence. BioTechniques. 2002, vol. 32, No. 4, pp. 940-944 and 946-949, especially p. 942, 943, and 946, Table 1, Figure 3.
Kutyavin et al. 3′-Minor groove binder-DNA probes increases sequence specificity at PCR extension temperatures. Nucleic Acids Research. 2000, vol. 28, No. 2, pp. 655-661, especially p. 656, 2nd column, 3rd paragraph; Figure 4; Figure 5; p.658, 2nd column, bottom paragraph to p. 659, 1st column, 1st paragraph; and Figure 7.

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