Alkaline cellulase variants

Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...

Reexamination Certificate

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Details

C435S209000, C435S252300, C435S320100, C510S320000, C536S023200

Reexamination Certificate

active

07033981

ABSTRACT:
The present invention relates to alkaline cellulase variants having an amino acid sequence which exhibits at least 95% homology with the amino acid sequence of SEQ ID NO: 2, wherein said alkaline cellulase variant is mutated to include at least one substitution at (a) position 10, (b) position 16, (c) position 22, (d) position 33, (e) position 39, (f) position 76, (g) position 109, (h) position 242, (i) position 263, (j) position 308, (k) position 462, (l) position 466, (m) position 468, (n) position 552, (o) position 564, or (p) position 608 in SEQ ID NO: 2, or at a position corresponding thereto, wherein said alkaline cellulase variant has alkaline cellulase activity. The present invention also relates to a gene encoding the variant; a vector containing the gene; a transformant containing the vector; and a detergent composition containing the alkaline cellulase variant.

REFERENCES:
patent: 4945053 (1990-07-01), Ito et al.
patent: 50-28515 (1975-09-01), None
patent: 60-23158 (1985-06-01), None
patent: 6-30578 (1994-04-01), None
patent: 10-313859 (1998-12-01), None
patent: 2000-210081 (2000-08-01), None
patent: WO 98/12307 (1998-03-01), None
Accession No. AAR26021 (see the enclosed sequence search alignment between Applicants' SEQ ID No.: 2 and Accession No. AAR26021, and notes therein).
Derwent Abstracts, Database Geneseq Online !, Database Accession No. AAG80267, 1 page, XP-002251476, JP 2001-231569, Aug. 28, 2001.
Derwent Abstracts, Database Genesq Online !, Database Accession No. AAR26021, 1 page, XP-002251477, JP 04-190793, Jul. 9, 1992.
K. Horikoshi, et al., Alkalophilic Microorganisms, pp. 126-129, “Enzymes of Alkalophilic Bacteria”, 1982.
Derwent Abstracts, Database Geneseq Online !, Database Accession No. AAG80267, 1 page, XP-002251476, JP 2001-231569, Aug. 28, 2001.
Derwent Abstracts, Database Geneseq Online 1, Database Accesion No. AAR26021, 1 page, XP-002251477, JP 04-190793, Jul. 9, 1992.

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