Aids assay

Details Aids assay Aids assay

1988-06-09

1992-04-28

Nucker, Christine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 79, 435 792, 435974, 436 58, 436531, 436825, 436826, G01N 33569

Patent

active

051088916

ABSTRACT:
An assay which is responsive to the levels of anti-p24 antibodies in serum samples is provided which comprises the steps of: (a) forming a test mixture comprising the sample and an antigen solution containing free p24 antigen within a predetermined concentration range: (b) incubating the test mixture under conditions whereby anti-p24 antibodies from the sample, if any, can react with the free p24 antigen to form antibody-antigen complexes; (c) determining the concentration of free p24 antigen remaining in the test mixture after the incubation; (d) determining the concentration of free p24 antigen in the antigen solution; and (e) calculating the difference between the concentration of free p24 antigen in the antigen solution and the concentration of freee p24 antigen in the test mixture after the incubation.
The difference calculated in step (e) represents the "binding capacity" of the serum sample for p24 antigen. This parameter is shown to serve as an accurate and convenient measure of a patient's level of anti-p24 antibodies. In certain preferred embodiments, the anti-p24 antibody assay is integrated with a p24 antigen assay so that declining anti-p24 antibody levels in combination with rising p24 antigen levels can be readily monitored. This combination has been found to generally indicate a poor prognosis for HIV-infected patients.

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