Agarose composition, aqueous gel and method of making same

Details Agarose composition, aqueous gel and method of making same Agarose composition, aqueous gel and method of making same

1979-02-07

1981-09-22

Lovering, Richard D.

Compositions

Preservative agents

Anti-corrosion

204180G, 252408, B01J 1300, B01D 5702

Patent

active

042909112

ABSTRACT:
A finely-divided solid blend of (1) purified agarose having an electroendosmosis value (-Mr) below 0.10 with (2) a water-soluble gum which by itself is soluble in boiling water without gelling, free from charged groups and from electroendosmosis, and soluble in water to form a solution of gum having at a concentration no greater than 10% by weight a viscosity of at least 10 cps. at 25.degree. C. The blend is soluble in water to form a gel having no electroendosmosis and useful as a medium for carrying out isoelectric focusing.

REFERENCES:
patent: 2466146 (1949-04-01), Baker
patent: 2508726 (1950-05-01), Ramstad et al.
patent: 3527712 (1970-09-01), Renn et al.
patent: 3767787 (1973-10-01), Segal
patent: 3783118 (1974-01-01), Hjerten
patent: 4146454 (1979-03-01), Haber
Johansson et al.: "Electrophoresis, Crossed Immunoelectrophoresis, and Isoelectric Focusing . . . ", Anal. Biochem., vol. 59, pp. 200-213 (1974).
Quast: "The Electroosmotic Flow in Agarose Gels . . . ", J. Chromat., vol. 54, pp. 405-412 (1971).
Weise et al.: Progress in Isoelectric Focusing and Isotachophoresis, Ed. Righetti, North Holland Publ. Co., 1975, pp. 93-98.
Saravis: Abstract Entitled Immunoelectrophoresis Publ. in Conjunction with 1978 Electrophoresis Conference, Apr. 19-21, 1978.

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