Active truncated form of the RNA polymerase of flavivirus

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S005000

Reexamination Certificate

active

08030047

ABSTRACT:
The isolation and purification of two domains from a from aflavivirusis provided. Each domain can function independently. Moreover, one domain codes for a sequence that provide polymerase activity. A process for screening possible modulators of the polymerase activity of an isolated and purified polypeptide fromflavivirusis also disclosed.

REFERENCES:
patent: 6949353 (2005-09-01), Romette et al.
patent: 7459297 (2008-12-01), Romette et al.
patent: 2654113 (1991-05-01), None
patent: WO01/60847 (2001-08-01), None
Guyatt et al (Journal of Virological Methods 92:37-44, 2001).
Grun et al (Journal of Virology 61:3641-3644, 1987).
U.S. Appl. No. 12/255,967, filed Oct. 22, 2008, Romette, et al.
Marie-Pierre Egloff, et al., “An RNA Cap (Nucleoside-2′-O-)-Methyltransferase in theFlavivirusRNA Polymerase NS5: Crystal Structure and Functional Characterization”, EMBO J. 2002, 21: pp. 2757-2768.
Michael Mc Lai, “RNA Polymerase as an Antiviral Target of Hepatitis C Virus”, Antiviral Chemistry & Chemotherapy, 2001,12, Suppl. 1: pp. 143-147.
Michelle P. Walker, et al., “HCV RNA-Dependent RNA Polymerase as a Target for Antiviral Development”, Current Opinion in Pharmacology 2002, 2: pp. 1-7.
A. Mlinaric, et al., “Screening of Selected Plant Extracts for In Vitro Inhibitory Activity on HIV-1 Reverse Transcriptase (HIV-1 RT)”, Pharmazie 2000, 55: pp. 75-77.
Renate Mentel, et al., “Inhibition of Adenovirus DNA Polymerase by Modified Nucleoside Triphosphate Analogs Correlate with their Antiviral Effects on Cellular Level”, Med Microbiology Immunology, 2000,189: pp. 91-95.
Fabien Zoulim, “Therapy of Chronic Hepatitis B Virus Infection: Inhibition of the Viral Polymerase and other Antiviral Strategies”, Antiviral Res; 1999, 44: pp. 1-30.
Meitian Wang, et al., “Non-nucleoside Analogue Inhibitors Bind to an Allosteric Site on HCV NS5B Polymerase: Crystal Structures and Mechanism of Inhibition” The Journal of Biological Chemistry, 2003, 278: pp. 9489-9495.
Steven S. Carroll, et al., “Inhibition of Hepatitis C Virus RNA Replication by 2′-Modified Nucleoside Analogs”, The Journal of Biological Chemistry, 2003, 278: pp. 11979-11984.
Scott G. Baginski, et al., “Mechanism of Action of aPestivirusAntiviral Compound”, Proc Natl Acad Sci USA, 2000, 97: pp. 7981-7986.
G. Campiani, et al., “Non-nucleoside HIV-1 Reverse Transcriptase Inhibitors: Synthesis and Biological Evaluation of Novel Quinoxalinylethylpyridylthioureas as Potent Antiviral Agents”, Antiviral Chemistry & Chemotherapy, 2000,11: pp. 141-155.
Anastasya L. Khandazhinskaya, et al., “Carbocyclic Dinucleoside Polyphosphonates: Interaction with HIV Reverse Transcriptase and Antiviral Activity”, Journal of Medical Chemistry, 2002, 45: pp. 1284-1291.
Erik De Clercq, “Antiviral Drugs: Current State of the Art”, Journal of Clinical Virology, 2001: pp. 73-89.
Hélène Malet, et al., “Crystal Structure of the RNA Polymerase Domain of the West Nile Virus Non-Structural Protein 5”, Journal of Biological Chemistry, 282: pp. 10678-10689, (2007).
Thai Leong Yap, et al., “Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution”, Journal of Virology, 81: pp. 4753-4765, (2007).
Brooks et al., “The interdomain region of dengue NS5 protein that binds to the viral helicase NS3 contains independently functional importin beta 1 and importin alpha/beta-recognized nuclear localization signals,” The Journal of Biological Chemistry, vol. 277, No. 39 (2002), 36399-36407.
Johansson et al., “A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin—and the viral helicase, NS3,” Journal of General Virology, 82: 735-745 (2001).
Yaegashi et al., “Partial sequence analysis of cloned dengue virus type 2 genome,” Gene, 46: 257-267 (1986).
Result 8, rpr, (2006).
Sequence search result 3 from .rag, (2006).
Sequence search results 1-5 from .rpr; search results 1-60 from .rup, (2006).
See SCORE sequence search result 14.rag (search done Jun. 1, 2006).
Bartholomeusz et al., “Synthesis of dengue virus RNA in vitro: initiation and the involvement of proteins NS3 and NS5,” Arch Virol, 128, pp. 111-121 (1993).
Tan et al (Virology 216:317-325, 1996).
Johansen et al (Journal of General Virology 82:735-745, 2001).
Vasudevan et al ( Farmaco 56:33-36, 2001).

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