Activating hydroxyl groups of polymeric carriers using 4-fluorob

Details Activating hydroxyl groups of polymeric carriers using 4-fluorob Activating hydroxyl groups of polymeric carriers using 4-fluorob

1990-08-13

1993-04-20

Naff, David M.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

435178, 435179, 435180, 435803, 436529, 436530, 436531, 436824, 530813, 530814, 530815, C07K 1700, C12N 1112, C12N 1110, G01N 33544

Patent

active

052044513

ABSTRACT:
The invention relates to a method of binding biologically active organic ligands to hydroxyl groups of polymeric carriers. The method involves bringing 4-fluorobenzenesulfonyl Chloride into reactive contact with the hydroxyl groups of polymeric carriers in such a manner to form sulfonate groups in place of the hydroxyl groups. The ligand is then brought into reactive contact with the hydroxyl groups of polymeric carriers to replace the sulfonate groups reacted with the organic ligand. The polymeric carrier containing the bound ligand can be used to isolate a biologically active material from a heterogeneous solution.

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