Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se
Reexamination Certificate
2007-07-03
2007-07-03
Bui, Phuong T (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
C435S320100, C435S419000, C435S468000, C536S024100, C800S278000, C800S300000
Reexamination Certificate
active
11075808
ABSTRACT:
The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5×SSC at 42° C.; The expression vector, also, includes a coding sequence located downstream of the promoter. Additionally, the method includes introducing the expression vector into a plant cell to obtain a transgenic plant; wherein the promoter activity is lower in mature seeds or root bases than in plant tissues other than mature seeds or root bases. The present invention also provides transformants containing a promoter; the promoter includes any of the DNAs (a) to (c) described above.
REFERENCES:
Kim et al. (Plant Molecular Biology, 24:105-117, 1994).
Sasaki et al. (NCBI, GenBank, Sequence Accession No. AP005841, pp. 1-53, Published in NCBI database in 2002).
Li et al. (Plant Physiol., 100:662-668, 1992).
Ichikawa Hiroaki
Kaku Koichiro
Kawai Kiyoshi
Nakamura Hidemitsu
Shimizu Tsutomu
Birch & Stewart Kolasch & Birch, LLP
Bui Phuong T
Kumar Vinod
Kumiai Chemical Industry Co. Ltd.
National Institute of Agrobiological Sciences
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