Acaricidal compositions and process for preparing same

Details Acaricidal compositions and process for preparing same Acaricidal compositions and process for preparing same

1992-01-30

1994-05-17

Robinson, Douglas W.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

424 93N, 424 93C, C12N 120

Patent

active

053126220

DESCRIPTION:

BRIEF SUMMARY
FIELD OF THE INVENTION

The invention relates to acaricidal compositions containing as active ingredient the fermentation broth of one of two bacterium strains deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary (hereinafter abbreviated: NCAIM) under the identification Nos. 001083 and 001086 on May 25, 1989, or the fermentation broth of their mixed micropopulation. The invention further relates to the cell-free fermentation broth of said bacterium strains or a concentrate of the cell-free or non cell-free fermentation broth thereof or a solidified form of the cell-free or non cell-free fermentation broth thereof, which may be in a sterilized form, optionally together (in admixture) with additive(s). The invention further relates to a process for the preparation of both strains and their mixed micropopulation.
The invention also relates to the bacillus strain deposited at NCAIM under the identification No. 001083 and the mixed micropopulation of both strains, respectively.
The invention further relates to the agricultural use of both bacterium strains or their mixed micropopulation.
From the two bacteria mentioned above, the bacillus strain deposited at NCAIM under the identification No. 001083 is novel whereas the pseudomonas deposited under the identification No. 001086 is known. The mixed microA4635-67-TF/KmO population of both bacteria is also novel.
The composition according to the invention is used e.g. for protection against parasitic mites living on Apis mellifera (honey-bee), preferably against Varroa jacobsoni mites (against varroatosis).


BACKGROUND OF THE INVENTION

It is known that Varroa jacobsoni is a mite parasitically living on Apis mellifera and which usually sucks at the faeces of bee-grubs by its sucking mandible and the pellicle of the pupae; but most willingly, it taps the haemolymph of the growing bees. As a result of the infection and in proportion thereto various distortioned evolution forms appear, the bees become weakened, become susceptible to other diseases and finally they perish.
The infection spreads to all important melliferious areas causing substantial losses. After the first infection, such losses appear in a surge-like manner, at the beginning in every third and fourth year and later in every second or third year. The death of the bee colony resembles an intoxication in most cases.
Several ways of protection against varroatosis are known:
1) Treatment or fumigation, respectively of the bee hives with various ethereal oils;
2) Application of various synthetic organic active agents, e.g.: Chemical Abstracts World Patent Index (hereinafter: WPI) Acc. No. 86-212705/337; British Crop Protection Council, Registry Number (hereinafter: Reg. No.) 116-29-07 and dicofol (Reg. No. 115-32-2) (WPI Acc. No. 86-037330/06); 4,4'-dibromo-benzylate (WPI Acc. No. 85-290439/47); 85-224091/37); and 87.130028/19).
Compositions containing synthetic organic active ingredients are applied by dusting (atomizing) or fumigation on the hives. Such commercialized compositions (which have, however, not been authorized in all countries) are e.g. Mitac EC (200 g/l) and Mitac WP (250-500 g/kg) containing amitrase as active ingredient; and Ectodex EC (50 g/l) (The Pesticide Manual 8th Ed., 1987; The British Crop. Protection Council, entry number 330). Their use is possible only at an appropriate external temperature (above 10.degree. C.).
According to some suppositions, in the case of treatments carried out at the wrong time or with too high a frequency, damaging side products can be accumulated in the honey which may be harmful to both the bee-keeper and the honey as well.
3) Use of attracting and repelling agents (attractants and repellents):
4) Other methods of protection, e.g.


SUMMARY OF THE INVENTION

During our investigations aimed at the protection of bees against mites it has been found that the fermentation broth of one or two bacterium strains deposited at NCAIM under the identification Nos. 001083 and 001086 or the fermentation broth of their

REFERENCES:
Bergy's Manual of Determinative Bacteriology, Eighth Ed; R. E. Buchanan et al, p. 226, Williams and Wilkins, 1974.
The Prokaryotes, A Handbook on Habitats, Isolation and Identification, of Bacteria, Mortimer P. Starr et al, vol. I, p. 689, Springer-Verlag (1981).

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