A3 adenosine receptor, DNA, and uses

Details A3 adenosine receptor, DNA, and uses A3 adenosine receptor, DNA, and uses

1993-08-02

1995-08-15

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Algae, media therefor

435 6, 435 691, 4353201, 536 235, 536 2431, C12N 1512

Patent

active

054418836

ABSTRACT:
The present invention relates to a novel mammalian adenosine receptor. The invention is directed toward the isolation, characterization and pharmacological use of the rat A3 adenosine receptor, the gene corresponding to this receptor, a recombinant eukaryotic expression construct capable of expressing the rat A3 adenosine receptor in cultures of transformed eukaryotic cells and such cultures of transformed eukaryotic cells that synthesize the rat A3 adenosine receptor. The invention also provides methods for screening adenosine-receptor agonists and antagonists in vitro using preparations of the rat A3 adenosine receptor from such cultures of eukaryotic cells transformed with a recombinant eukaryotic expression construct comprising the rat A3 adenosine receptor gene.

REFERENCES:
Ribeiro & Sebastiao, 1986, "Adenosine receptors and calcium: basis for proposing a third (A3) adenosine receptor", Prog. Neurobiol. 26: 179-209.
Stiles, 1986, "Novel adenosine receptors", Trends Pharmacol. Sci. 7: 486-490.
Williams, 1987, "Purine receptors in mammalian tissues: pharmacology and functional significance", Ann. Rev. Pharmacol. Toxicol. 27: 315-345.
Ramkumar et al., 1988, "Adenosine receptors: clinical implications and biochemical mechanisms", Prog. Drug. Res. 32: 195-247.
Fredholm & Dunwiddie, 1988, "How does adenosine inhibit transmitter release?", Trends Pharmacol. Sci. 9: 130-134.
Nakata, 1989, "Purification of A1 adenosine receptor from rat brain membranes", J. Biol. Chem. 264: 16545-16551.
Libert et al., 1989, "Selective amplification and cloning of four new members of the G protein-coupled receptor family", Science 244: 569-72.
Olah et al., 1990, "Purification and characterization of bovine cerebral cortex A1 adenosine receptor", Arch. Biochem. Biophys. 283: 440-446.
Maenhaut et al., 1990, "RDC8 codes for an adenosine A2 receptor with physiological constitutive activity", Biochem. Biophys. Res. Commun. 173: 1169-1178.
Ali et al., 1990, "Activation of phospholipase C via adenosine receptors provides synergistic signals for secretion in antigen-stimulated RBL-2H3 cells. Evidence for a novel adenosine receptor", J. Biol. Chem. 265: 745-753.
Zhou et al., 1990, "Cloning and expression of human and rat D1 dopamine receptors", Nature 347: 76-80.
Sebastiao et al., 1990, "The inhibitory adenosine receptor at the neuromuscular junction and hippocampus of the rat: antagonism by 1,3,8-substituted xanthines", Br. J. Pharmacol. 100: 55-62.
Stiles, 1990, "Adenosine receptors and beyond: molecular mechanisms of physiological regulation", Clin. Res. 38: 10-18.
Nakahata et al., 1991, "Adenosine inhibits histamine-induced phosphoinositide hydrolysis mediated via pertussis toxin-sensitive G protein in human astrocytoma cells", J. Neurochem. 57: 963-969.
Meyerhof et al., 1991, "Molecular cloning of a novel putative G-protein coupled receptor expressed during rat spermiogenesis", FEBS Lett. 284: 155-160.
Oliveira et al., 1991, "Solubilized rat brain adenosine receptors have two high-affinity binding sites for 1,3-dipropyl-8-cyclopentylxanthine", J. Neurochem. 57: 1165-1171.
Libert et al., 1991, "The orphan receptor cDNA RDC7 encodes an A1 adenosine receptor", EMBO J. 10: 1677-1682.
Mahan et al., 1991, "Cloning and expression of an A1 adenosine receptor from rat brain", Molecular Pharmacol. 40: 1-7.
Reppert et al., 1991, "Molecular cloning and characterization of a rat A1-adenosine receptor that is widely expressed in brain and spinal cord", Molec. Endo. 5: 1037-1048.
Lin et al., 1991, "2-Chloroadenosine decreases tyrosylprotein sulfotransferase activity in the Golgi apparatus in PC12 cells. Evidence for a novel receptor", J. Biol. Chem. 266: 14457-14463.

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