Process for isolating Cu, Zn-superoxide dismutase from aqueous s

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435192, 435814, 435815, C12N 902

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active

043906281

ABSTRACT:
Cu,Zn-superoxide dismutase (SOD) is isolated from aqueous solutions containing said enzyme together with accompanying proteins by chromatography of the solution at a pH of 4.7 to 5.0 on a cation exchange resin of the same polarity as SOD in the pH range used. As cation exchange resin may particularly be used carboxymethyl celluloses, cross-linked dextrans substituted with carboxymethyl groups or sulfopropyl groups or cross-linked agaroses substituted with carboxymethyl groups.
The process lends itself to use on an industrial scale and provides a high yield of pure SOD.

REFERENCES:
patent: 3758682 (1973-09-01), Huber et al.
Goscin et al., Biochem. Biophys. Acta, vol. 289, pp. 276-283, (1972).

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