Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1981-11-10
1983-06-28
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435192, 435814, 435815, C12N 902
Patent
active
043906281
ABSTRACT:
Cu,Zn-superoxide dismutase (SOD) is isolated from aqueous solutions containing said enzyme together with accompanying proteins by chromatography of the solution at a pH of 4.7 to 5.0 on a cation exchange resin of the same polarity as SOD in the pH range used. As cation exchange resin may particularly be used carboxymethyl celluloses, cross-linked dextrans substituted with carboxymethyl groups or sulfopropyl groups or cross-linked agaroses substituted with carboxymethyl groups.
The process lends itself to use on an industrial scale and provides a high yield of pure SOD.
REFERENCES:
patent: 3758682 (1973-09-01), Huber et al.
Goscin et al., Biochem. Biophys. Acta, vol. 289, pp. 276-283, (1972).
De Forenede Bryggerier A/S
Shapiro Lionel M.
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