Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-12-16
2000-01-18
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1534, C07H 2104
Patent
active
060156732
ABSTRACT:
The invention relates to methods and compositions that are useful for detecting deficiencies in dihydropyrimidine dehydrogenase (DPD) levels in mammals including humans. Cancer patients having a DPD deficiency are at risk of a severe toxic reaction to the commonly used anticancer agent 5-fluorouracil (5-FU). Claimed are DPD genes from human and pig, methods for detecting the level of nucleic acids that encode DPD in a patient, and nucleic acids that are useful as probes for this purpose. Also claimed are methods for expressing DPD in heterologous organisms. Expression vectors that employ a DPD nucleic acid as a selectable marker are also claimed. This selectable marker functions in both prokaryotes and eukaryotes.
REFERENCES:
Cheng et al, "Molecular cloning of dihydropyrimidine dehydrogenase", Clin. Pharm. Therap. 55(2):188, Feb. 1994.
Gaedgk et al, "Characterization of the microsomal epoxide hydrolase gene in patients with anticonvulsant adverse drug reactions", Pharmacogenetics 4(3):142-153 Abstract Only, Jun. 1994.
Lu et al, "Dihydropyrimidine dehydrogenase activity in human peripheral blood mononucleasr cells and liver: Population characteristics, newly identified patients and clinical implication in 5-fluorouracil therapy", Cancer Research 53:5433-5438, Nov. 1993.
Fernandez-Salguero Pedro
Gonzalez Frank J.
Fredman Jeffrey
The United States of America as represented by the Department of
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