Method for the isolation and expression of a gene which codes fo

Details Method for the isolation and expression of a gene which codes fo Method for the isolation and expression of a gene which codes fo

Filed on

1991-05-22

Date issued

1994-03-22

Examiner

Wax, Robert A.

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Enzyme , proenzyme; compositions thereof; process for...

SubSubClass

Hydrolase

Other Related Categories

435 691, 435 698, 435 711, 435 712, 4351723, 4352523, 43525233, 4353201, 4352542, 43525423, 536 232, C12N 1558, C12N 1570, C12N 1581

Type

Patent

Status

active

Patent number

052963667

Description

ABSTRACT:
The present invention relates to the field of biotechnology and genetic engineering and in particular a novel nucleotide sequence which codes for a streptokinase, as well as the recombinant DNA obtained therefrom which is used for the transformation of various host organisms.
The present invention is based on the isolation of a new gene which codes for streptokinase from Streptococcus equisimilis of type C (strain ATCC-9542) and the cloning and expression thereof in prokaryotic (E. coli) and eukaryotic (Pichia pastoris) hosts, for which it includes the vehicles of expression which contain the genetic sequences of said gene, as well as the microorganisms transformed with these vectors capable of producing streptokinase.
The protein obtained thereby can be used in clinical medicine as a therapeutic agent, in the treatment of disorders such as thromboembolic obstructions including coronary thrombosis.

REFERENCES:
Hagenson, M. J. et al. "Expression of Streptokinase in Pichia pastoris Yeast" Enzyme. Microb. Technol. 11: 650-656 (Oct. 1989).
S. K. Park et al. "The Leader Sequence of Streptokinase . . . " Biochem. Biophys. Res. Commun. 174(1) 282-286 (Jan. 1991).

Affiliated with

Also associated with

Rating

Method for the isolation and expression of a gene which codes fo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the isolation and expression of a gene which codes fo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the isolation and expression of a gene which codes fo will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-436196