Primer set capable of specifically amplifying a target...

Details Primer set capable of specifically amplifying a target... Primer set capable of specifically amplifying a target...

2005-12-23

2009-06-30

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S024320, C536S024330

Reexamination Certificate

active

07553626

ABSTRACT:
Provided are a primer set for amplifying a target sequence specific toBordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, andStreptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.

REFERENCES:
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5525718 (1996-06-01), Ohashi et al.
patent: 5744305 (1998-04-01), Fodor et al.
patent: 5830654 (1998-11-01), Milliman
patent: 6001564 (1999-12-01), Bergeron et al.
patent: 6582908 (2003-06-01), Fodor et al.
patent: 2003/0175709 (2003-09-01), Murphy et al.
patent: 2004/0072242 (2004-04-01), Hunter et al.
patent: 2006/0046246 (2006-03-01), Zeng et al.
Buck et al. Design strategies and performance of custom DNA sequencing primers. BioTechniques (1999) 27(3): 528-536.
Rossau et al. DNA probes forBordetellaspecies and a colorimetric reverse hybridization assay for the detection ofBordetella pertussis. Molecular and Cellular Probes (1992) 6(4): 281-289.
GenBank Accession No. U10876 forMoraxella catarrhalis16S rRNA, Jun. 29, 1994 [online], [retrieved on Jun. 7, 2008], retrieved from the Internet: <URL: www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=506691>.
GenBank Accession No. L06108 forChlamydia pneumoniae16S rRNA, Sep. 21, 1993 [online], [retrieved on Jun. 7, 2008], retrieved from the Internet: <URL: //www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=174111>.
GenBank Accession No. X73402 forLegionella pneumophila16S rRNA, Oct. 1, 1993 [online], [retrieved on Jun. 7, 2008], retrieved from the Internet: <URL: www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=405770>.
GenBank Accession No. M29061 forMycoplasma pneumoniae16S rRNA, Apr. 27, 1993 [online], [retrieved on Jun. 7, 2008], retrieved from the Internet: <URL: www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=175479>.
GenBank Accession No. AJ608938 forStreptococcus pneumoniae16S rRNA (May 17, 2004 [online], [retrieved on Jun. 8, 2008], retrieved from the Internet: <URL: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id =38520888>.
Stone et al. Detection of rRNA from four respiratory pathogens using an automated Qβ replicase assay. Molecular and Cellular Probes (1996) 10(5): 359-370.
Barry, Tom et al.; “A General Method to Generate DNA Probes for Microorganisms”; Bio/Technology; vol. 8; pp. 233-236; 1990.
Jantos, Christian et al.; “Rapid Detection ofChlamydia pneumoniaeby PCR-Enzyme Immunoassay”; Journal of Clinical Microbiology, pp. 1890-1894; vol. 36, No. 7; Jul. 1998.
Greisen, K. et al.; “PCR Primers and Probes for the 16S rRNA Gene of Most Species of Pathogenic Bacteria, Including Bacteria found in Cerebrospinal Fluid”; Journal of Clinical Microbiology, pp. 335-351; vol. 32(2); Feb. 1994.
Grimm, Dorothee et al.; “Specific Detection ofLegionella pneumophila: Construction of a New 16S rRNA-Targeted Oligonucleotide Probe”; Appl Environ Microbiol, Jul. 1998, pp. 2686-2690, vol. 64, No. 7.
Ouverney, Cleber et al.; “Combined Microautoradiography-16S rRNA Probe Technique for Determination of Radioisotope Uptake by Specific Microbial Cell Types in Situ”; Appl Environ Microbiol, Apr. 1999, pp. 1746-1752, vol. 65, No. 4.
Yogev, D. et al.; “Distinction of Species and Strains of Mycoplasmas (Mollicutes) by Genomic DNA Fingerprints with an rRNA Gene Probe”; Journal of Clinical Microbiology, Jun. 1998; pp. 1198-1201; vol. 26; No. 6.
Bootsma, Hester et al.; “Analysis ofMoraxella catarrhalisby DNA Typing: Evidence for a Distinct Subpopulation Associated with Virulence Traits”; Journal of Infectious Diseases; 2000;181: pp. 1376-1387.

Affiliated with

Also associated with

Rating

Primer set capable of specifically amplifying a target... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Primer set capable of specifically amplifying a target..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Primer set capable of specifically amplifying a target... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4095010