pH dependent ion exchange material, solid substrate having...

Details pH dependent ion exchange material, solid substrate having... pH dependent ion exchange material, solid substrate having...

2006-05-09

2008-08-26

Gulakowski, Randy (Department: 1796)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S287200, C521S032000, C525S054200, C518S722000, C526S085000

Reexamination Certificate

active

07416845

ABSTRACT:
Provided are a pH dependent ion exchange material having a carboxyl group, an amino group, and a polyethylene oxide moiety, which is used for isolating a nucleic acid, a solid substrate having the material immobilized on its surface, and a method of isolating a nucleic acid using the material or the solid substrate. The pH dependent ion exchange material has at least two monomers selected from the group consisting of M0, M1, M2, M3 and M4 represented by the following formulae, provided that the pH dependent ion exchange material has at least one monomer selected from the group consisting of M1 and M2 and at least one monomer selected from the group consisting of M3 and M4:whereinA is a base selected from the group consisting of —NH(CH2)nNH2and —NH(CH2)nY, wherein n is an integer from 1 to 10 and Y is an aromatic base in which at least one of ring atoms is nitrogen,B is —(CH2CHO)nOR2, wherein n is 1-20 and R2is a C1-10 alkyl group or a protecting group, andthe pH dependent ion exchange material having a degree of polymerization of 2-30,000.

REFERENCES:
patent: 6310199 (2001-10-01), Smith et al.
patent: 6806362 (2004-10-01), Smith et al.
patent: 2001/0018513 (2001-08-01), Baker

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