Nucleic acids encoding pseudomonas hop proteins and use thereof

Details Nucleic acids encoding pseudomonas hop proteins and use thereof Nucleic acids encoding pseudomonas hop proteins and use thereof

2006-11-21

2006-11-21

Kubelik, Anne (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

Higher plant, seedling, plant seed, or plant part

C800S279000, C536S023700, C424S093200, C435S320100, C435S252300

Reexamination Certificate

active

07138569

ABSTRACT:
The present invention relates to isolated nucleic acid molecules encoding a type III—secreted bacterial protein capable of modifying a cell death pathway in a plant cell. One aspect of the present invention involves an isolated nucleic acid molecule having a nucleotide sequence that encodes the HopPtoD2 protein ofPseudomonas syringaepv.syringaeDC 3000. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. The nucleic acid molecules of the present invention can be used to impart disease resistance to a plant and to make a plant hypersusceptible to colonization by nonpathogenic bacteria.

REFERENCES:
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Bauer et al. 1999, Acta Hort. 489:301-304.
Espinosa et al, 2003, Molec. Microlbiol. 49:377-387.
Collmer et al., “Pseudomonas syringaeHrp Type III Secretion System and Effector Proteins,”PNAS97(16):8770-8777 (2000).
Alfano et al., “ThePseudomonas syringaeHrp Pathogenicity Island has a Tripartite Mosaic Structure Composed of a Cluster of Type III Secretion Genes Bounded by Exchangeable Effector and Conserved Effector Loci That Contribute to Parasitic Fitness and Pathogenicity in Plants,”PNAS97(9):4856-4861 (2000).
Fouts et al., “Genomewide Identification ofPseudomonas syringaepv. Tomato DC3000 Promoters Controlled by the HrpL Alternative Sigma Factor,”PNAS99(4):2275-2280 (2002), with supplemental material available online at www.pnas.org.
Petnicki-Ocwieja et al., “Genomewide Identification of Proteins Secreted by the Hrp Type III Protein Secretion System ofPseudomonas syringaepv. Tomato DC3000,”PNAS99(11):7652-7657 (2002), with supplemental material available online at www.pnas.org.

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