Modified chitin-binding domain and use thereof

Details Modified chitin-binding domain and use thereof Modified chitin-binding domain and use thereof

2006-01-10

2006-01-10

Carlson, Karen Cochrane (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S200000, C530S300000, C530S350000, C514S012200

Reexamination Certificate

active

06984505

ABSTRACT:
Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.

REFERENCES:
patent: 5187262 (1993-02-01), Raikhel et al.
patent: 5643758 (1997-07-01), Guan et al.
patent: 5834247 (1998-11-01), Comb et al.
patent: 5928917 (1999-07-01), Kilburn et al.
patent: 5962289 (1999-10-01), Kilburn et al.
patent: 6124117 (2000-09-01), Kilburn et al.
patent: 6174700 (2001-01-01), Haynes et al.
patent: WO 00/47751 (2000-08-01), None
patent: WO 01/57183 (2001-08-01), None
Yamagami et al. Biosci. Biotech. Biochem. 61(11): 1819-1825 (1997).
LaVallie and McCoy, Curr. Opin. Biotechnol., 6501-506 (1995).
Xu, et al., Methods Enzymol. 326:376-418 (2000).
Van Dyke, et al. Gene 111:99-104 (1992).
Smith and Johnson, Gene 67:31-40 (1998).
Watanabe, et al., J. Bacteriol. 176:4465-4472 (1994).
Jolles, et al. Chitin and Chitinases, Birkhauser Verlag, Basel (1999).
Hashimoto, et al. J. Bacteriol. 182:3045-3054 (2000).
Watanabe, et al. J. Bacteriol. 172:4017-4022 (1990).
Alam, et al. J. Ferment. Bioeng. 82:28-36 (1996).
Nagai, et al., Nature 309:810-812 (1984).
New England Catalog 2002/2003, p. 163.
Steel, et al. J. Immunol. 145:3917-3923 (1990).
Evans, et al., Biopolymers 51:333-342 (1999a).
Cantrell, et al. Proc. Natl. Acad. Sci, USA 82:6250-6254 (1985).
Mingsheng, et al., J. Biotechnol. 11:157-162 (1995).
Evans, et al. J. Biol. Chem. 274:18359-18363 (1999b).
Yamamoto, et al., Nature 319:230-234 (1986).
Doherty, et al., Proc. Natl. Acad. Sci. USA 96:10869-10874 (1999).
Chong, et al., Gene 192:271-281 (1997).
Studier, et al., Methods Enzymol. 185:60-89 (1990).
Bradford, Anal. Biochem., 72:248-254 (1976).
Ikegami et al., Solution Structure of the Chitin-binding Domain ofBacillus circulansWL-12 Chitinase A1, The Journal of Biological Chemistry, vol. 275, No. 18:13654-13661(2000).
Boller et al., Chitinase in Bean Leaves:Induction by Ethylene, Purification, Properties, and Possible Function, Planta, vol. 157:22-31 (1983).

Affiliated with

Also associated with

Rating

Modified chitin-binding domain and use thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modified chitin-binding domain and use thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modified chitin-binding domain and use thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3524891