Method of monitoring reagent delivery in a scanning spectrophoto

Details Method of monitoring reagent delivery in a scanning spectrophoto Method of monitoring reagent delivery in a scanning spectrophoto

1989-12-01

1991-11-26

Lacey, David L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

436 56, 436 69, 436164, 436166, 436172, 436800, 356 39, G01N 2177, N01N 2178

Patent

active

050681818

ABSTRACT:
A method for measuring the concentration of a reagent in a reaction mixture including the steps of: adding dye to a reagent until the dye is at a given concentration in the reagent; mixing the reagent with a reaction mixture comprising a sample which reacts with the reagent to form a reaction product; measuring the formation of reaction product at a first spectral region; measuring the concentration of dye in the reaction mixture at a second spectral region in which the dye has an optical characteristic such as absorption or fluorescence, the second spectral region being different from the first spectral region; and determining the concentration of the reagent in the reaction mixture based on the concentration of dye measured.
In a further aspect of the invention there is provided a reagent containing a dye useful in the above method.

REFERENCES:
patent: 4204839 (1980-05-01), Wu et al.
patent: 4329149 (1982-05-01), Schoonover et al.

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