Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-03-23
1994-05-03
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436 94, 435 912, C12Q 168, G01N 3350
Patent
active
053087518
ABSTRACT:
A method for simultaneously sequencing both strands of a target DNA is provided. The method involves "shifting" one or both strands of the target DNA by addition or deletion of one or more nucleotides to one or both strands of the duplex to produce shifted DNA. Both strands of the shifted DNA duplex and the target DNA are sequenced and the sequences of the shifted and unshifted target DNA are compared. Since portions of the sequenced strands of the target and shifted DNA are identical, all or a portion of sequence of the target DNA can be determined.
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Miller Robert L.
Ohkawa Tihiro
General Atomics
Parr Margaret
Schreiber David
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