Reagent for assaying endotoxin

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435184, 435962, 436518, 436529, 436530, 436531, 5303882, 53038826, 5303891, G01N 33569, G01N 33543, G01N 33579

Patent

active

056058063

ABSTRACT:
The present invention provides a reagent for assaying an endotoxin which comprises limulus amoebocyte lysate and an antibody to (1 .fwdarw. 3)-.beta.-D-glucan sensitive factor or a reagent which comprises a lysate substantially free from (1 .fwdarw. 3)-.beta.-D-glucan sensitive factor. The reagent of the present invention makes it possible to assay an endotoxin originating from gram-negative bacteria contained in a biological sample such as blood, urine and cerebrospinal fluid at an extremely high sensitivity without being affected by (1 .fwdarw. 3)-.beta.-D-glucan.

REFERENCES:
patent: 4414336 (1983-11-01), Watanabe et al.
patent: 5155032 (1992-10-01), Tanaka et al.
patent: 5202253 (1993-04-01), Esmon et al.
patent: 5266461 (1993-11-01), Tanaka

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