Method for testing the toxicity of chemicals using hyperactivate

Details Method for testing the toxicity of chemicals using hyperactivate Method for testing the toxicity of chemicals using hyperactivate

1995-02-13

1996-10-29

Corbin, Arthur L.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435 32, A01N 102, C12Q 118

Patent

active

055695809

ABSTRACT:
A method is provided for the in vitro testing of chemicals to determine roductive toxicity using hyperactivated rabbit spermatozoa, and a method is provided for the in vitro production of said rabbit spermatozoa of hyperactivated motility useful in said testing. Spermatozoa are incubated in a simple salts medium in air at 22.degree. to 37.degree. C. Hyperactivated motility develops in one-half to one hour. Motility parameters are then measured using motion analysis systems and models for the classification of spermatozoa based on motility. Inhibition of hyperactivated motility of spermatozoa by exposure to chemicals may be used as an in vitro method of assessing the reproductive consequences of exposure of males to chemicals.

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Young et al. Molecular Reproduction and Development 33: 347-356 (1992).
Brackett et al. Biology of Reproduction 12: 260-274 (1975).
GIBCO BRL Catalogue & Reference Guide.

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