Method for producing oligosaccharide

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435207, 435254, 435911, 435913, 435918, 536 11, 536123, 536124, C12P 1904, C12N 938, C12N 114, C07H 300

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049578600

ABSTRACT:
The present invention provides a method of producing oligosaccharides by the reaction between lactose and .beta.-galactosidase from aspergillus oryzae in which an concentration of lactose in an enzyme reaction mixture is between 50 and 90% (w/v) and a reaction temperature is within the range of 55.degree. C. to the temperature at which the .beta.-galactosidase in the reaction mixture is inactivated. This invention therefore makes it possible to obtain the sugar mixture containing high-purity oligosaccharides, small amounts of unreacted lactose and by produced monosaccharides.

REFERENCES:
patent: 3592739 (1971-07-01), Sternberg
patent: 3919049 (1975-11-01), Kiuchi et al.
patent: 4435389 (1984-03-01), Mutai et al.
Chemical Abstracts, Nakano et al., Nov. 23, 1987, p. 365, #193924(p).
M. Dixon et al.: "Enzymes", 2nd Edition, 1971, pp. 36,37, 144-151, 466, 467, Longman, London, GB pp. 27, 148, 467.

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