Method for the separation of extracellular amylase and protease

Details Method for the separation of extracellular amylase and protease Method for the separation of extracellular amylase and protease

1983-03-30

1985-04-02

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435219, 435814, 435816, 435832, C12N 926, C12N 950, C12R 107

Patent

active

045088250

ABSTRACT:
Extracellular protease and amylase coproduced during the fermentation of a microorganism capable of producing them are separated by the addition of polyethyleneglycol and a cationic epihalohydrin/polyamine copolymer or dextran polymer to the fermentation medium and allowing the polymers to phase separate to form a protease rich phase and an amylase rich phase.

REFERENCES:
patent: 4144130 (1979-03-01), Kula et al.
Hustedt et al., Biotechnology and Bioengineering, vol. XX, pp. 1989-2005, (1978).
Albertsson, Biochemistry, vol. 12, No. 13, (1973), pp. 2525-2530.

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