Methods for producing polypeptide metal binding sites and compos

Details Methods for producing polypeptide metal binding sites and compos Methods for producing polypeptide metal binding sites and compos

1993-06-14

1997-10-21

Feisee, Lila

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 7021, 4351721, 4351885, 536 2553, 536 243, 5303871, 530400, 514 6, 930 25, C12N 1513, C07K 1600, A61K 39395

Patent

active

056795483

ABSTRACT:
The present invention describes methods for producing metal binding sites on polypeptides, and particularly for producing metal binding sites within the CDR regions of immunoglobulin heavy or light chains that are displayed on the surface of filamentous phage particles. The invention also describes oligonucleotides useful for preparing the metal binding sites, and human monoclonal antibodies produced by the present methods.

REFERENCES:
Barbas et al. PNAS 89 p. 4457-4461 1992.
Roberts et al. PNAS 87 pp. 6654-6658 1990.
Iverson et al. Science vol. 249:659 1990.
Stansfield et al. Tibtech 12: 275 1994.

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