Identification, characterization, and method of production of a

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435198, 435271, 435814, C12N 120, C12N 920

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active

050631608

ABSTRACT:
A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10.+-.0.5; (ii) an optimum temperature for activity of about 45.degree. to 55.degree. C.; (iii) an optimum pH for stability of about 7.0.+-.0.5; (iv) a molecular weight as measured by gel permeation chromotagraphy of about 8.8.times.10.sup.4 ; and (v) chemical stability for at least 30 days in the presence of the surfactants. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase.

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Watanabe, N. et al., (1977), Agric. Biol. Chem. 41(8), 1353-1358.
Gel Filtration Theory and Practice (1979), Pharmacia Fine Chemicals, p. 42.
Ralston-Barrett, E., N. J. Palleronie, and M. Doudoroff, Inter. J. System. Bacteriol, 26(4): 421-426 (1976).
ATCC Catalogue of Bacteria and Phages, (17th ed), 1989, p. 170.

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