Bacterial screening system with enhanced shelf life

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 26, 435810, C12Q 104, C12Q 132

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active

047286083

ABSTRACT:
A tetrazolium-based system for testing for the presence of organisms or cells in a specimen includes in one embodiment a mixture of a substrate and an indicator system, wherein the overall system is buffered to a pH above 8.0, and in a preferred embodiment at about a pH of 9.0 when mixed. The substrate solution and indicator solution are not mixed until shortly before use. The indicator solution is buffered at a pH of approximately 3.3, thereby enhancing the shelf life. The substrate may consist of glucose with at least one amino acid in a buffered solution formulated such that, by mixing equal parts with the indicator solution, a pH above 8, preferably 9.0 results.

REFERENCES:
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patent: 3732147 (1973-05-01), Fosker et al.
patent: 4026767 (1977-05-01), Shih et al.
patent: 4120755 (1978-10-01), Pierre et al.
patent: 4129483 (1978-12-01), Bochner
patent: 4556634 (1985-12-01), Misaki et al.

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