Polynucleotides and their use for detecting enterococci and stre

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 435 912, 536 231, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2104, C07H 2102

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active

060542691

ABSTRACT:
Polynucleotides and oligonucleotides for identification of species of the Streptococcus genus and the Enterococcus genus are provided. The polynucleotides and oligonucleotides are useful as probes and primers. Polypeptides expressed by the polynucleotides and oligonucleotides are useful for the preparation of monoclonal and polyclonal antibodies that recognize the polypeptides.

REFERENCES:
Garnier et al, Genbank records SMU69164, SMU69165, SSU69167, SSU69168, SOU69166, SGU69163, Feb. 1997.
Dutka-Malen et al, "Sequence of the vanC gene of Entetrococcus gallinarum BM4174 encoding a D-alanine:D-alanine ligase related protein necessary for vancomycin resistance", Gene 112:53-58, 1992.
Kajimura et al, "Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization", GATA 7(4):71-79, Aug. 1990.
Navarro et al, "Analysis of genes encoding a D-alanine-D-alanine ligase related enzymes in Entterococcus casseliflavus and Enterococcus flavescens", Antimicrobial Agents and Chemotherapy 38(8):1788-1793, Aug. 1994.
Evers et al, "Sequence of the vanB and ddl genes encoding D-alanine:D-lactate and D-alanine:D-alanine ligases in vancomycin resistant enterococcus faecalis V583", Gene 140:97-102, 1994.
Evers et al, "Evolution of structure and substrate specificity in D-alanine:D-alanine ligases and related enzymes", J. Mol. Evolution 42(6):706-12 Abstract Only, Jun. 1996.
Invitrogen catalog, p. 35, 1992.
Stratagen catalog, p. 39, 1988.
Garnier et al, "Identification of clinically relevant viridans group streptococci to the species level by PCR", J. Clin. Microbiol. 35(9):2337-2341, Sep. 1997.

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