Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1994-12-08
1998-05-12
Benzion, Gary
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800250, 800DIG56, 47 58, 47DIG1, 536 271, 536 241, 4351723, 4351721, 435424, 435430, 935 86, C12H 1500, C12H 1505, A01H 106, A01H 400
Patent
active
057508682
ABSTRACT:
Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and structural genes capable of acting in a cascading fashion to produce a reversible effect on a plant phenotype. A first genetic construct comprising (i) an operator that is capable of controlling expression of a dominant negative gene, (ii) a dominant negative gene that, when expressed in a plant disrupts pollen formation or function, (iii) a tissue specific promoter that regulates the expression of a gene encoding a DNA-binding protein which binds to the operator and activates transcription, and (iv) a gene encoding a DNA binding region and an activating domain, causes male sterility in a plant. In particular, the present invention relates to the use of a DAM-methylase gene as a dominant negative gene and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene.
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Albertsen Marc C.
Cigan Andrew M.
Benzion Gary
Pioneer Hi-Bred International , Inc.
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