Composition for the colorimetric determination of metals

Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids

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436 84, 422 61, G01N 3348, G01N 3352

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active

044079621

ABSTRACT:
An improved colorimetric reagent is disclosed, which is adapted to the determination of the iron content in blood serum. The formulation comprises only two aqueous solutions: one of these comprises a buffer, a soluble salt of an alkali metal or an alkaline earth metal, Chromazurol B, a cationic or nonionic surfactant, whereas the other solution comprises a complexing agent and possibly the same ingredients of the chromogenic reagent for hindering the formation of the disturbing complex between chromazurol B, the surfactant and the iron: the complexing agent is a polycarboxylic acid or a salt thereof.

REFERENCES:
patent: 4224034 (1980-09-01), Denney et al.
patent: 4308027 (1981-12-01), Ceriotti
Clinical Chemical Acta, vol. 114, pp. 287-290, (1981), Tabacco et al.
Clinical Chemical Acta, vol. 94, pp. 115-119, (1979), Garcic, A.
Microchemical Journal, vol. 23, pp. 28-41, (1978), Vekhande, C. et al.
Deutsch Gesundheitswes, vol. 22, pp. 1686-1688, (1967), Schmidt, G.

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