Method for determining the activity of the angiotensin-convertin

Details Method for determining the activity of the angiotensin-convertin Method for determining the activity of the angiotensin-convertin

1981-12-14

1983-10-04

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 24, C12Q 134, C12Q 136

Patent

active

044079443

ABSTRACT:
A method for determining the activity of an angiotensin-converting enzyme is disclosed comprising the steps of mixing a liquid containing (i) a synthetic substrate X-hippuryl-L-dipeptide having the formula ##STR1## wherein X represents OH, NH.sub.2 or N(CH.sub.3).sub.2, and when R.sub.1 is H, R.sub.2 is H, and when R.sub.1 is ##STR2## R.sub.2 is CH.sub.2 CH(CH.sub.3).sub.2, (ii) hippuricase and (iii) 4-aminoantipyrine, with a liquid containing an angiotensin-converting enzyme; measuring colorimetrically the concentration of the quinonimine dye which is formed by adding an oxiding agent to the above mixture; and calculating the activity of the angiotensin-converting enzyme from the concentration of the quinonimine dye.

REFERENCES:
patent: 4115374 (1978-09-01), Ryan et al.
patent: 4260682 (1981-04-01), Ryan et al.
patent: 4292404 (1981-09-01), Kasahara et al.

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