Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1994-01-12
1995-08-08
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041801, 2041823, 204299R, 204301, G01N 2726, G01N 27447
Patent
active
054395739
ABSTRACT:
An electrophoretic gel elutor and concentrator includes a semi-permeable collecting membrane having a continuously diminishing cross-section terminating in an apex, and a supporting frame of matching configuration. This apparatus is useful in electrophoretic extraction of biological samples which have been previously cut from a separation gel. The apparatus is used with standard electrophoresis equipment including a buffer tank, electrodes, and a power source. Due to the high effective cross-section of the collecting membrane, electrophoretic elution can be carried out quickly and efficiently. Due to the continuously diminishing cross-section to the apex of the collecting membrane, the biological sample is advantageously concentrated and extractions of 99% theoretical yield from a separated gel fragment is possible.
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Litman Richard C.
Niebling John
Starsiak Jr. John S.
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