Microbial expression of a gene for human growth hormone

Details Microbial expression of a gene for human growth hormone Microbial expression of a gene for human growth hormone

1982-03-09

1986-08-05

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435172, C12N 120, C12N 1500

Patent

active

046043594

ABSTRACT:
Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illustrative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.

REFERENCES:
patent: 4332892 (1982-06-01), Ptashne et al.
Li et al., J. Am. Chem. Soc., vol. 92, pp. 7608 and 7609 (1970).
Roberts et al., Proc. Natl. Acad. Sci. U.S.A., vol. 76, No. 2, pp. 760-764 (Feb. 1979).
Martrial et al., Science, vol. 205, pp. 602-607 (Aug. 10, 1979).
Itakura et al., Science, vol. 198, pp. 1056-1063 (Dec. 9, 1977).

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