Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Liposomes
Patent
1995-06-07
1998-03-17
Ketter, James
Drug, bio-affecting and body treating compositions
Preparations characterized by special physical form
Liposomes
435199, 4353201, 536 241, 536 251, A61K 9127
Patent
active
057283990
ABSTRACT:
An improved soluble molecular complex for targeting a polynucleotide to a specific cell is disclosed. The molecular complex comprises (a) a polynucleotide (b) a carrier made up of a polynucleotide binding agent and a cell-specific binding agent which binds to a surface molecule of the cell and is internalized into an endosome, and (c) a bacterial component or fragment thereof which lyses the endosome and causes the polynucleotide to be released into the cytoplasm of the cell. In a preferred embodiment of the invention the polynucleotide binding agent is polylysine, the cell-specific binding agent is an asialoglycoprotein, and the bacterial component is listeriolysin O. The disclosed soluble molecular complex and methods of use can be used therapeutically to deliver genes and antisense polynucleotides to specific cells in vivo.
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Carmichael Ellen
Spitalny George L.
Wu Catherine H.
Wu George Y.
Zhang Ying
Ketter James
Remillard Jane E.
TargeTech, Inc. a wholly owned subsidiary of The Immune Response
University of Conn.
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