Increased fermentation yield of expressed protein

Details Increased fermentation yield of expressed protein Increased fermentation yield of expressed protein

1988-08-25

1991-05-07

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435255, 435256, 935 37, C12N 1567

Patent

active

050136500

ABSTRACT:
A galactose-regulated yeast strain expresses a desired protein in improved yield by growing the transformed yeast cells in culture in fed-batch mode and replacing the culture medium with fresh medium before adding the galactose to induce expression of the foreign protein.

REFERENCES:
Fieschko, J., et al., Biotech. and Bioeng., 29, 1113-1121 (1987).
Blanch, H., et al., (editors), Comprehensive Biotechnology, vol. 3, Pergamon Press, Oxford, 1985, pp. 442-443.
Stepien et al., Synthesis of a Human Insulin Gene GENE, 24 (1983) 289-297.
Kramer et al., Regulated Expression of a Human Interferon Gene in Yeast: Control by Phosphate Cone. or Temp. P.N.A.S., 81 (Jan. 1984) 367-370.
Miyanohara et al., Expression of Hepatitis B Surface Antigen in Yeast P.N.A.S., 80 (Jan. 1983) 1-5.

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