Lactam-containing compositions and methods useful for the hybrid

Details Lactam-containing compositions and methods useful for the hybrid Lactam-containing compositions and methods useful for the hybrid

1991-12-13

1994-12-27

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

540451, 540463, 540485, 540526, 540527, 546243, 548547, C12Q 168

Patent

active

053765290

ABSTRACT:
This invention relates to novel methods for the release of nucleic acids from cells in complex biological samples or specimens to prepare and make available the nucleic acid material present for a hybridization assay or for extraction. Novel methods for hybridization of nucleic acids are also presented. In particular methods are described for isolating nucleic acid from a sample containing a complex biological mixture of nucleic acid and non-nucleic acids wherein the sample is combined with a hybridization medium comprising a lactam which promotes and enables nucleic acid pairing when complementary nucleic acid is introduced. The lactam is preferably about 5 to about 70% of the hybridization medium and is most preferably 2-pyrrolidone, N-ethyl-2-pyrrolidone, N-cyclohexyl-2-pyrrolidone, N-dodecyl-2-pyrrolidone, N-methyl-2-pyrrolidone, N-hydroxyethyl-2-pyrrolidone, N-methyl-2-piperidone, 2-.epsilon.-caprolactam, N-methyl-2-caprolactam, 2-piperidone or N-(4-hydroxybenzyl)pyrrolidone.

REFERENCES:
patent: 4302204 (1981-11-01), Wahl et al.
patent: 4483920 (1984-11-01), Gillespie et al.
patent: 4652517 (1987-03-01), Scholl et al.
patent: 4830969 (1989-05-01), Holmes

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