Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-02-28
1996-03-26
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 75, 436528, 436537, 436829, C12Q 168, G01N 3353, G01N 33542, G01N 33544
Patent
active
055019536
ABSTRACT:
Membranes of liposomes fixing biotin on membrane surfaces thereof can be lysed by a mixed agent comprising a surfactant and avidin. This mechanism can be applied to immunoassay for determining an analyte, e.g. antigen or antibody, wherein liposomes encapsulating a marker and fixing biotin on membrane surfaces thereof are lysed depending on hindrance of an avidin-biotin binding reaction by a specific reaction caused by the analyte.
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WO86/04682, published Aug. 14, 1986.
Litchfield et al, Clinical Chemistry, vol. 30, No. 9, 1984, pp. 1441-1445.
Muzykantov et al, FEBS Letters, vol. 280, No. 1, 1991, pp. 112-114.
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Plant et al "Generic Liposome Reagent for Immunoassays" Anal. Biochem. 176: 420-426 1989.
Czerkinsky "Antibody-Secreting Cells" Chapter 1.3 in Methods of Enzymatic Analysis ed H. U. Bergmeyer 1986 VCH pp. 23-44, Esp. p. 39.
Fujita Minoru
Kida Masaaki
Duffy Patricia A.
Scheiner Toni R.
Wako Pure Chemical Industries Ltd.
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