Process for quantitatively lysing liposomes and a process for de

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 71, 435 75, 436528, 436537, 436829, C12Q 168, G01N 3353, G01N 33542, G01N 33544

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active

055019536

ABSTRACT:
Membranes of liposomes fixing biotin on membrane surfaces thereof can be lysed by a mixed agent comprising a surfactant and avidin. This mechanism can be applied to immunoassay for determining an analyte, e.g. antigen or antibody, wherein liposomes encapsulating a marker and fixing biotin on membrane surfaces thereof are lysed depending on hindrance of an avidin-biotin binding reaction by a specific reaction caused by the analyte.

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WO86/04682, published Aug. 14, 1986.
Litchfield et al, Clinical Chemistry, vol. 30, No. 9, 1984, pp. 1441-1445.
Muzykantov et al, FEBS Letters, vol. 280, No. 1, 1991, pp. 112-114.
Muzykantov et al, Biochem. J., vol. 273, 1991, pp. 393-397.
Plant et al "Generic Liposome Reagent for Immunoassays" Anal. Biochem. 176: 420-426 1989.
Czerkinsky "Antibody-Secreting Cells" Chapter 1.3 in Methods of Enzymatic Analysis ed H. U. Bergmeyer 1986 VCH pp. 23-44, Esp. p. 39.

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