Method for stabilizing polyacrylamide gels

Synthetic resins or natural rubbers -- part of the class 520 ser – Synthetic resins – Processes of preparing a desired or intentional composition...

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524 57, 524 58, 525 542, C08L 500

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051590492

ABSTRACT:
A method for preparing stabilized, rehydratable polyacrylamide gels, wherein the stabilizers employed include substituted monosaccharides where the hydroxyl group of the carbon 1 is substituted with a non-charged group comprising an .alpha.-methyl group or an oligosaccharide having from 2 to about 8 repeating units which can be the same or different. These rehydratable gels can be stored for extended periods of time at ambient temperatures, and can be rehydrated without loss of structural or functional integrity to be used for electrophoretic separation of proteins and nucleic acids.

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Frey et al., "Preparation of Rehydratable Polyacrylamide Gels and their Application in Ultrathin-Layer Isoelectric Focusing" Electrophresis, vol. 7, pp. 28-40 (1986).
Gelfi et al., "Swelling Kinetics of Immobiline Gels for Isoelectric Focusing" Electrophoresis, vol. 5, pp. 257 and 262.
Allen et al., "Enzyme and Antibody Detection Following Isoelectric Focusing on Ultrathin-Layer Rehydrated Polyacrylamide Gels" Acta Histochem. Cytochem., vol. 19, No. 5, pp. 637-645.
Allen et al., "Tailoring DNA Resolution in Polyacrylamide Gels with Electrochemistry and Pulsed Constant Power" Electrophoresis '89, p. 91 (1989).

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