Method of detecting antibodies

Details Method of detecting antibodies Method of detecting antibodies

1987-01-06

1989-08-08

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

436545, 436804, 436811, 435 7, G01N 33539

Patent

active

048552429

ABSTRACT:
A method for determining the quantity of an antibody in a sample, the method having the steps of: (1) providing a labelled antigen to the antibody; (2) contacting the labelled antigen with the sample in solution to form a labelled antigen-antibody complex; (3) providing an agent for precipitating the complex; (4) mixing the solution containing the labelled antigen-antibody complex with the precipitating agent to produce a precipitate and a supernatant; the supernatant containing labelled antigen and the precipitate containing the labelled antigen-antibody complex and uncomplexed labelled antigen; and (5) measuring the quantity of label in the precipitate or the supernatant in a manner substantially independent of the amount of uncomplexed labelled antigen in the precipitate.

REFERENCES:
patent: 3995019 (1976-11-01), Jerome
Kurtz et al., 18 Diabetologia 147 (1980).
Palmer et al., 222 Science 1337 (1983).
Salih et al., 41 Chem.-Biol. Interactions 169 (1982).
Miyamoto et al., 17 (1) Jap. J. Med. 15 (1978).
Osterman et al., 34 Steroids 575 (1979).
Henquin et al., 10 Diabetologia 61 (1974).
Ionescu-Tirgouiste et al., 27 Diab. 592 (1984).
Goldman et al., 27 Diab. 653 (1978).
Versijp et al., 15 Horm. Metabol. Res. 456 (1983).

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