Method for screening an expression cDNA clone bank for the detec

Chemistry: analytical and immunological testing – Involving iga – igd – ige – or igm

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

436512, 5303885, G01N 33563, C07K 1616

Patent

active

058375503

ABSTRACT:
The invention relates to a method by which expression cDNA clone banks are screened by means of IgE antibodies derived from the sera of allergic individuals, or by means of monoclonal or polyclonal antibodies. This type of screening is used for the detection of polynucleotides whose open-reading frames code for allergenically active proteins. These proteins are characterized in that their biological activity as allergens is equal to that of plant allergens occurring in nature.

REFERENCES:
patent: 4721668 (1988-01-01), Jones, III et al.
Fang et al., Proc. Natl. Acad. Sci. (USA) 85:895-899 (1988).
Breiteneder et al., Int. Arch. Allergy Appl Immunol 87:17-24 (1988).
Lauzurica et al., "Olive (Olea Europea) Pollen Allergens-I. Immunochemical Characterization by Immunoblottin, Crie and Immunodetection by a Monoclonal Antibody," Mol. Immunol. 25(4): 329-335 (1988).
Sutton et al., "Detection of IgE and IgG-Binding Proteins After Electrophoretic Transfer from Polyacrylamide Genes," J. Immunol. Meth. 52:183-194 (1982).
Helfman, D., and S. Hughes, 1987, "Use of Antibodies to Screen cDNA expression Libraries Prepared in Plasmid Vectors", Methods of Enzymology 152:451-458.
Hatton et al., "Identification of cDNA Clones Representing Group I Pollen Allergens from Kentucky Bluegrass", in Allergy and Molecular Biology, A. El Shami and T. Merrett eds., Pergamon Press, Oxford. (Jan. 1989).
Davis et al., Microbiology (J.B. Lippincott, Philadelphia) 4th Edition (1990).
Ipsen, H. and Hansen, O.C. in: Epitopes of Atopic Allergens. Sehon, A.H., Kraft, D., Kunkel, G. (eds) (1990) UCB, Brussels, Belgium, pp. 3-8.
Rumpold, et al. In: Epitopes of Atopic Allergens. Sehon, A.H., Kraft, D., Kunkel, G. (eds) (1990) UCB, Brussels, Belgium, pp.; 26-28.
Valenta, et al. In: Epitopes of Atopic Allergens. Sehon, A.H., Kraft, D., Kunkel, G. (eds) (1990) UCB Brussels, Belgium, pp. 73-76.
Florvaag, E. et al. Int. Arch. Allergy Appl. Immunol. 75:300-308 (1984).
Florvaag, E. et al. Int. Arch. Allergy Appl. Immunol. 67:49-56 (1982).
Jarolim, et al. Int. Arch. Allergy Appl. Immunol. 90:54-60 (1989).
Hatton, T.W., Hill, R.D., Ekramoddoullah, A.K.M., Kisil, F.T. and Sehon, A.H., "Molecular Cloning of Kentucky Bluegrass (KBF) Pollen Allergens," J. 58, siehe den ganzen Artikel, Siete p. 183.
Lutck, H.A. et al. EMBO J. 6(1):43-48 (1987).
Thomas et al. In: Epitopes of Atopic Allergens. Sehon, A.H., Kraft, D., and Kunkel, G. (eds), 1990. UCB Institute of Allergy, Brussels.
Nagai, K. et al. Nature 309:810-812 (1987).
Stinson, J.R. et al. Plant Physiol. 83:442-447 (1987).
Walter, M.H. et al., Proc. Natl. Acad. Sci. USA 85:5546-5550 (Aug. 1988).
Rogers, B. et al., International Publication WO 90/11293, published 4 Oct. 1990, entitled: "Allergenic proteins from ragweed and uses therefore".
Valenta, R. et al. J. Allergy Clin.Immunol. 88(6):889-894 (1991).
Stratagene Cloning Systems 1992 Product Catalog, Cloning Vectors: pBluescript II, pp. 30-31.
Stratagene Technical Profile of pBluescript II Phagemid.
Tchang et al. J. Biol. Chem. 263(32):16849-16855 (1988).
Crawford et al. Proc. Natl. Acad. Sci. USA 83:8073-8076 (1986).
Reed et al. Proc. Natl. Acad. Sci. USA 85:7661-7665 (1988).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for screening an expression cDNA clone bank for the detec does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for screening an expression cDNA clone bank for the detec, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for screening an expression cDNA clone bank for the detec will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-883717

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.