Class II restriction endonuclease KspI and a process for obtaini

Details Class II restriction endonuclease KspI and a process for obtaini Class II restriction endonuclease KspI and a process for obtaini

1989-03-30

1990-12-04

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435195, 435196, 435814, 435815, 435822, 435259, C12N 922, C12N 914, C12N 916, C12R 101

Patent

active

049753762

ABSTRACT:
The present invention provides a restriction endonuclease which recognizes palindromic sequences ##STR1## where C* is methylated, and cleaves these sequences at the position indicated by the arrows. This endonuclease is preferably from a microorganism of the genus Kluyvera. The present invention also provides a process for obtaining this new restriction endonuclease and a method for using the endonuclease.

REFERENCES:
patent: 4064011 (1977-12-01), Mayer et al.
patent: 4588689 (1986-05-01), Kado et al.
patent: 4668631 (1987-05-01), Obayashi et al.
patent: 4693978 (1987-09-01), Stetter et al.
patent: 4746609 (1988-05-01), Bolton et al.

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