Nucleic acid sequence encoding FLP recombinase

Details Nucleic acid sequence encoding FLP recombinase Nucleic acid sequence encoding FLP recombinase

1997-11-18

1999-07-27

Robinson, Douglas W.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

800278, 435419, 47 58, 536 231, C12N 504, C12N 1529, A01H 500, A01H 510

Patent

active

059293012

ABSTRACT:
A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.

REFERENCES:
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Gordon-Kamm et al. Plant Cell. 1990. vol. 2: 603-618.
Ausubel et al. Short Protocols in Molecular Biology. John Wiley & Sons. 1989.
Murray, Elizabeth E., et al., "Codon Usage in Plant Genes," Nucleic Acids Research, 1989, pp. 477-498, vol. 17, No. 2.
Perlak, Frederick J., et al., "Modification of the Coding Sequence Enhances Plant Expression of Insect Control Protein Genes," Proc. Natl. Acad. Sci. USA, Apr. 1991, pp. 3324-3328, vol. 88.
Lloyd, Alan M., et al., "Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum", Mol. Gen. Genet., 1994, pp. 653-657, vol. 242.
Lyznik, Leszek A., et al., "Heat-inducible expression of FLP gene in maize cells", Plant Journal, 1995, pp. 177-186, vol. 8(2).

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