Expression of a gram-positive bacteria replicon

Details Expression of a gram-positive bacteria replicon Expression of a gram-positive bacteria replicon

1997-10-21

1999-07-27

McKelvey, Terry

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C12P 2102

Patent

active

059288973

ABSTRACT:
A recombinant vector capable of replicating in Gram positive bacteria and containing: a) a nucleotide concatenation I of Bacillus thuringiensis with a size of about 2.6 kb between sites BalI-HpaI, or any fragment included in said concatenation as long as its allows replication of the recombinant vector when under the control of functional promoter in Gram positive bacteria, or any sequence which hybridizes with the complementary sequence of concatenation I or the above mentioned fragment under highly stringent conditions, and b) at least on DNA sequence of interest inserted in phase with the above mentioned concatenation.

REFERENCES:
Lereclus et al, FEMS Microbiology Letters, vol. 60: pp. 211-218 (1989).
Lereclus et al, FEMS Microbiology Letters, vol. 49: pp. 417-422 (1988).

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