Method for preparing mutant genes

Details Method for preparing mutant genes Method for preparing mutant genes

1995-12-15

1999-07-27

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435440, 435441, 435449, 435448, C12Q 168

Patent

active

059288663

ABSTRACT:
This invention provides A method for preparing mutant genes, which comprises the steps of constructing a recombinant plasmid DNA by inserting a gene fragment into a plasmid DNA having a unidirectional origin, introducing the recombinant plasmid DNA into host cell lacking DNA error-correcting function to transform the host cell, and culturing the transformant cell in a condition that any mutations of the inserted gene is detectable. According to the present invention, diverse mutant genes can be prepared in a short period of time.

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Armstrong et al., "Excision repair and gene prientation modulate the strand specificity of UV mutagenesis in a plasmid-borne yeast tRNA gene", Environment. Mol. Mutagen., 25: 12-22, 1995.
Foster et al., "Interactions between epsilon, the proofreading subunit of DNA polymerase III, and proteins involved in the SOS response of Escherichia coli", Mol. Gen. Genet. 214:467-473, 1988.

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