Method of immobilizing and preserving an immunologically reactiv

Details Method of immobilizing and preserving an immunologically reactiv Method of immobilizing and preserving an immunologically reactiv

1992-06-09

1994-05-17

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

435 721, 435177, 435180, 435181, 435260, 436518, 436531, 436532, C12N 1100, C12N 1108, C12N 104, G01N 33545

Patent

active

053127447

ABSTRACT:
A method is provided for immobilizing and preserving an immunologically reactive antigen substance such as antigenic cells or non-cell bound antigens for use in solid-phase immunoassay. The antigen substance is adsorbed and crosslinked on a support and contacted with a protecting solution and containing sugar and an antiseptic substance such as NaN.sub.3. The antigen substance is preferably centrifugally contacted with the support to shorten the time for adsorption. Crosslinking is with a solution of 1.0 to 5.0% formaldehyde or 0.003 to 0.06% glutaraldehyde. The antigenic cells can be a blood component such as platelets.

REFERENCES:
patent: 4794096 (1988-12-01), Parham et al.
patent: 4891319 (1990-01-01), Roser
patent: 5030560 (1991-07-01), Sinor et al.
patent: 5124264 (1992-06-01), Imura et al.
Immunochemistry, Pergamon Press 1969, vol. 6, pp. 67-76.

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