Chemistry: analytical and immunological testing – Biospecific ligand binding assay – Utilizing isolate of tissue or organ as binding agent
Patent
1984-04-23
1985-05-28
Padgett, Ben R.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
Utilizing isolate of tissue or organ as binding agent
436510, 436515, 436530, 436531, 436543, 436804, 436808, 436809, 436811, 436546, 435 5, 435 7, 435194, 435239, 435810, 435948, 422 61, 424 86, 424 89, G01N 3354, G01N 3356
Patent
active
045201137
ABSTRACT:
This invention relates to the detection of antibodies in sera of AIDS and pre-AIDS patients and describes the biochemical and immunological analysis of antigens associated with the virus HTLV-III Human T-Cell Leukemia Virus. It is shown that antigens associated with the infection of human cells by this virus are specifically recognized by antibodies from AIDS patients. Specifically, HTLV-III isolated from AIDS patients and transmitted by cocultivation with an HT cell line is specifically detected by antibodies from human sera taken from AIDS patients. The method of detection of antibodies preferred is a strip radioimmunoassay (RIA) based on the Western Blot technique or an ELISA (an enzyme-linked immunosorbent assay) or an indirect immunofluorescence assay.
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Gallo Robert C.
Popovic Mikulas
Sarngadharan Mangalasseril G.
Moskowitz M.
Padgett Ben R.
Roberts, Jr. John S.
The United States of America as represented by the Secretary of
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