Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-12-06
1999-01-05
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536254, C12Q 168, C07H 2100
Patent
active
058561005
ABSTRACT:
Disclosed are: a part for assembling reaction vessels comprising a base plate with holes passing through said plate in the direction of thickness; a method for preparation of reaction vessels containing a reaction solution characterized in that the part is immersed in a reaction solution to introduce the reaction solution into the holes and then one of apertures of each hole is sealed with a membrane to complete said reaction vessels; and a plate comprises a base plate with holes passing through the plate in the direction of thickness and a membrane for sealing one of the apertures of each hole. The plate is used for purification of DNA sequencing samples using a pressure difference or an electrical potential, and transferring DNA sequencing samples to a separation/detection system. By the means or the method of the invention, reaction solutions can be easily introduced into a large number of wells in a short time, unreacted labeling substances and the like can be easily removed in a short time from a plurality of DNA sequencing samples, and a large number of DNA sequencing samples can be easily purified and/or transferred to electrophoresis capillaries in a short time.
REFERENCES:
patent: 5415758 (1995-05-01), Comeau
patent: 5560811 (1996-10-01), Briggs et al.
Cole PArmer Catalog, 1993.
Xiaohua C. Huang et al., "DNA Sequencing Using Capillary Array Electrophoresis", Analytical Chemistry (1992) 64:2149-2154, No. 18.
Jones W. Gary
Shoemaker Debra
The Institute of Physical and Chemical Research
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