Process for the isolation and purification of alpha-interferons

Details Process for the isolation and purification of alpha-interferons Process for the isolation and purification of alpha-interferons

1986-03-21

1989-07-04

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435811, 530351, 424 857, C12P 2100, C12P 2102, C07K 1300, C07K 1500

Patent

active

048450324

ABSTRACT:
The invention relates to a process for the isolation and purification of .alpha.-interferons by (a) dissolution of the interferon-containing crude product in guandine. HCl solution and precipitation by dilution with water and/or (b) by chromatography on an HIC column under renaturing conditions.

REFERENCES:
patent: 4405601 (1983-09-01), McEntire et al.
patent: 4476049 (1984-10-01), Kung
patent: 4485038 (1984-11-01), Chadha et al.
patent: 4569908 (1986-02-01), Mark et al.
Davey, M. W. et al., (1976), J. Biol. Chem. 251, 7620-7625.
Ernest Rinderknecht et al., "Natural Human Interferon-.gamma. Complete Amino Acid Sequence and Determination of Sites of Glycosylation", The Journal of Biological Chemistry, vol. 259, No. 11, Jun. 10, 1984, pp. 6790-6097.
Masateru Shin, Butyl-Toyopearl 650 as a New Hydrophobic Adsorbent for Water-Soluble Enzyme Proteins, Analytical Biochemistry, 138:259-261, (1984).

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