Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1996-12-06
1999-08-03
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435967, C12Q 100, C12Q 102, G01N 3353
Patent
active
059324368
ABSTRACT:
The invention provides a 6-day Daphnia reproductive bioassay for detecting and confirming the presence of a toxic substance in an aqueous sample, and/or for screening the substance as an endocrine disrupter. According to the assay, a test sample is brought into contact with at least three adult, oviporous Daphnia of a single clone under conditions of crowding and suboptimal growth conditions to cause stress and stimulate sexual reproduction. The preferred clone for use in the assay is Daphnia galeata-mendotae Wingra clone CDF-1. The bioassay is based upon the measurement of five endpoints that convey quantitative information about the biological activity of the substance: survivorship, numbers of female offspring, numbers of male offspring, number of resting eggs, and number of offspring that display developmental deformities. Also provided are kits for use in conducting the bioassay.
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Dodson Stanley I.
Girvin Kristin M.
Shurin Jonathan B.
Lankford , Jr. Leon B.
Tate Christopher R.
Wisconsin Alumni Research Foundation
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