Fluid introduction into a capillary by electroendosmosis

Details Fluid introduction into a capillary by electroendosmosis Fluid introduction into a capillary by electroendosmosis

1990-01-24

1991-12-17

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, B01D 5702

Patent

active

050732390

ABSTRACT:
Bulk fluid movement in a capillary column is achieved by creating electroendosmotic flow in a fluid passage outside the column, the fluid passage being joined to the column in a manner which causes the electroendosmotic flow to generate bulk flow in the column and yet permits an electric potential to be applied across the fluid passage and not the column. Small volume bulk fluid movement induced in this manner may be used to load sample into the column, whereas a larger induced volume may be used for flushing the column between runs. To alternate the bulk fluid movement with electrophoretic separations in the column, the column is arranged such that an electric potential can also be applied across the column without extending to the electroendosmotic fluid passage.

REFERENCES:
Everaerts, F. M. et al. "Simple Sampling Device for Capillary Isotachophoresis and Capillary Zone Electrophoresis" Journal of Chromatography 452 (1988) 615-622.
Bocek, P. et al, "Electric Sample Splitter for Capillary Zone Electrophoresis Journal of Chromatography", 320 (1985) 159-165.

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