Method for optical determination of the catalytic enzyme activit

Details Method for optical determination of the catalytic enzyme activit Method for optical determination of the catalytic enzyme activit

1991-06-04

1993-08-24

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435287, 435291, 435310, 435805, 435808, 436163, 436164, 422 55, 422 56, 422 57, 422 58, 422 8205, 422 8206, 422 8207, C12Q 100, C12M 100, C12M 134

Patent

active

052388093

ABSTRACT:
For optical determination of the catalytic enzyme activity of a sample by means of enzyme reactants which are split under the influence of the enzyme to be measured, the enzyme reactant is placed at the end of an optical fiber and brought into contact with the sample to be determined. The measurements are carried out by observing the rate of change in spectral characteristics of the enzyme reactant, or its reaction products, resulting from the enzyme reaction. The method permits measurements of undiluted blood and in-vivo determinations of enzyme activities.

REFERENCES:
patent: 4399099 (1983-08-01), Buckles
patent: 4803049 (1989-02-01), Hirschfeld et al.

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