Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-11-10
1999-12-07
Lee, Howard C.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 88, 536106, 536112, 536124, C08B 3700, C08B 1510, C08B 3702
Patent
active
059986069
ABSTRACT:
Novel methods are described to synthesize chromatography media. The methods describe a crosslinking procedure utilizing the catalytic effect of MnO(OH).sub.2 in obtaining a very rigid matrix. The mechanism of crosslinking is likely of free-radical type. This is also suggested by the concurrent functionalization steps which are known to be of free-mechanism and are greatly enhanced by MnO(OH).sub.2. The methods are simple and efficient as crosslinking and functionalization of the matrix takes place in the same step. High protein binding capacities are obtained along with an increased efficiency of the matrix. One particularly preferred process according to the present invention comprises: (1) reacting a polymeric carbohydrate matrix with an activating reagent which contains a halogen atom or epoxide and a double bond under alkaline conditions to generate an activated matrix; (2) reacting the activated matrix having double bonds inserted with a reagent or reagents producing MnO(OH).sub.2 ; and (3) activating the double bond in the presence of MnO(OH).sub.2 to crosslink the matrix and simultaneously derivatize the matrix with a halohydrin, carboxyl or sulfonate group.
REFERENCES:
patent: 3042677 (1962-07-01), Rogers et al.
patent: 3208994 (1965-09-01), Flodin
patent: 3507851 (1970-04-01), Ghetie et al.
patent: 3860573 (1975-01-01), Honkanen et al.
patent: 3959251 (1976-05-01), Porath et al.
patent: 4543363 (1985-09-01), Yanagihara
patent: 4663163 (1987-05-01), Hou et al.
patent: 4665164 (1987-05-01), Pernemalm et al.
patent: 4973683 (1990-11-01), Lindgren
M.S. Kharasch et al., "The Peroxide Effect in . . . Substitution of Bisulfite," J. Org. Chem. 3:175-192(1939) (Exhibit 9).
J. Porath et al., "Agar Derivatives for . . . Bead Form," J. Chromaotgr. 60:167-177 (1971) (Exhibit 10).
J.H. Helberger, "Zur Kenntnis . . . von Propansulton," Liebigs Ann. Chem. 588:71-78 (1954) (Exhibit 11).
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